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Antibody（phosphorylated proteins）｜一般社団法人　日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody（phosphorylated proteins）

【A】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04 WBIF IHCIP
Cell Signaling Techx400
#2914
4%PFA/PBS
rabbit

mouse oocyte
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04 WBIF IHCIP
Cell Signaling Techx400
#2914
ice cold Methanol
rabbit

mouse oocyte
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04 WBIF IHCIP
Cell Signaling Techx400
#2914
4%PFA/PBS
rabbit

HeLa
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04 WBIF IHCIP
Cell Signaling Techx400
#2914
ice cold Methanol
rabbit

HeLa
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04 WBIF IHCIP
Cell Signaling Techx400
#2914
ice cold Methanol
rabbit

MEF
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04 WBIF IHCIP
Cell Signaling Techx400
#2914
ice cold Methanol
rabbit

NIH3T3
phospho-AuroraA/AIK (T288)2014-07-11 WBIF IHCIP
Cell Signaling Techx400
#3091
ice cold Methanol
rabbit

HeLa
phospho-AuroraA/AIK (T288)2014-07-11 WBIF IHCIP
Cell Signaling Techx400
#3091
ice cold Methanol
rabbit

MEF
phospho-AuroraA/AIK (T288)2014-07-11 WBIF IHCIP
Cell Signaling Techx400
#3091
ice cold Methanol
rabbit

NIH3T3
phospho-Akt(Ser473)2011-10-17 WBIF IHCIP
Cell SignalingX500
#9271
rabbit
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
phospho-AuroraB (T232)2014-07-11 WBIF IHCIP
ROCKLANDx400
600-401-677
4%PFA/PBS
rabbit

HeLa

【C】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-CrkL (Tyr207)2011-10-17 WBIF IHCIP
Cell Signaling
#3181
rabbit
Poly

HeLa細胞

【E】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phopho-p44/42 MAPK (ERK1/2) (T202/Y204)2014-07-11 WBIF IHCIP
Cell Signaling Techx1000x400
#9101
4%PFA/PBS
rabbit
signal decreases from 3h after oocyte activation
mouse oocyte
phopho-p44/42 MAPK (ERK1/2) (T202/Y204)2014-07-11 WBIF IHCIP
Cell Signaling Techx400
#9101
ice cold Methanol
rabbit
signal decreases from 3h after oocyte activation
mouse oocyte
Phospho-Ezrin2011-09-27 WBIF IHCIP
Chemicon100
AB3832
rabbit
p
TCA>>4%PFA Ezrinに特異性はなくRadixin, Moesinも同様に認識。
Stains ezrin, radixin, and moesin.
HeLa

【M】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
MYPT1 pT850 (chick) / pT853 (human)2013-02-01 WBIF IHCIP
Upstate (Millipore)X1000
36-003
rabbit (poly)
cell lysate調製時に急速に脱リン酸化されるため、TCA処理またはSDS sample bufferによる調製を推奨 Samples should be directly prepared by TCA or SDS sample buffer because of the rapid dephosphorylation in preparation.
Phospho myosin light chain 2 (Thr18/Ser19)2011-09-27 WBIF IHCIP
Cell Signaling100
3674L
rabbit
p
1%PFA, 4%PFA
MDCK II, EpH4, MTD-1A, L
Phospho myosin light chain 2 (Ser19)2011-09-27 WBIF IHCIP
Cell Signaling100
3675S
mouse
p
1%PFA, 4%PFA
MDCK II, EpH4, MTD-1A, L
p-MLC(Thr18/Ser19)2011-09-27 WBIF IHCIP
Santa Cruz100
sc-12896
goat
p
1%PFA, 4%PFA
MDCK II

【P】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Phospho-PERK (Thr980)(16F8)2019-09-10 16F8WBIF IHCIP
Cell Signaling (CST)x1000
#3179
Rabbit
IgG

マウス大腸

【p】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-Smad22017-09-14 WBIF IHCIP
Invitrogen1/200
44-244G
4% PFA at 4oC over night
Rabbit
MeOH at -20oC over night

zebrafish embryos (8-10 hpf/hours post fertilization)
phospho-Smad1/5/82017-09-14 WBIF IHCIP
Cell Signaling1/200
9511
4% PFA at 4oC over night
Rabbit
MeOH at -20oC over night

zebrafish embryos (6 hpf/hours post fertilization)

【P】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Phosphorylated-Tyrosine(p-Tyr)2011-11-26 PY-99WBIF IHCIP
Santa Cruzx50
sc7020
4％PFA +0.1％Triton
mouse　IgG2b
IgG2b

Drosophila embryo, discs, nervous system

【S】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-Smad2/32011-09-22 WBIF IHCIP
Cell Signaling Techx250x50
3108
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
Mab
incubate w/ Phosphatase inhibitors
mouse E6.5 embryo. MEF
phospho-Smad1/5/82011-09-22 WBIF IHCIP
Cell Signaling Techx250x50
9511
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
poly?

mouse E6.5 embryo. MEF

【T】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-Tyr2011-10-17 WBIF IHCIP
Cell SignalingX500
#9411
mouse
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Mono, IgG1

ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞

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